Grid-based image stitching for microscopy on mobile + desktop.

Business requirements & challenges

Business requirements & challenges

Develop a grid-based image-stitching algorithm for microscopic images and deploy it on Android. Microscopic images have uniform backgrounds and partially uniform foregrounds, demanding a robust algorithm.

• Grid-based stitching across hundreds of partially overlapping tiles
• Algorithm must handle uniform foreground/background
• Deploy on Android for handheld scientific use
• Time-lapse capability across cell-culture studies

Solution

Solution

Automated microscope generates a grid of partially overlapping tiles. Algorithm matches features between neighbouring images irrespective of rotation, scale, or translation. Hundreds of overlapped tiles get assembled into a single mosaic image.

• Feature matching across rotated, scaled, and translated neighbours
• Median translation estimation for high-uncertainty matches
• Camera-angle estimation across the grid
• Minimum-spanning-tree assembly of the stitched image

Business benefits

Business benefits

• Enables time-lapse studies of cell cultures
• Differently exposed images normalised into single exposure for visual analysis
• Available on both desktop and mobile (handheld microscopy)